Journal of Health and Medical Sciences
Published: 22 September 2022
A Study on Distribution and Association of CD320, a Receptor for Active Vitamin B12, in Pregnant Mothers and their Newborns Cord Blood
M. V. Surekha, Gadhiraju S, K. Satyanarayana, Putcha Uday Kumar
Indian Council of Medical Research (India), Gandhi Hospital (India)
Download Full-Text Pdf
Keywords: CD320, Anaemia, Pregnant Mothers, Cord Blood, Vitamin B12, Active B12
Objective: Our objective was to study the levels and relation between CD320 receptors in anaemic mothers in their last trimester of pregnancy and their newborns after birth. Methods: Association between CD320, VitB12, active B12 levels in mothers with anaemia were analysed before they underwent labor and in blood collected from their newborns after labor. Regression analysis was performed in order to assess the relation between the mothers and their neonatal vitamin CD320 levels in relation to their Vit B12 status. Results: Among the 200 pregnant mothers recruited, 59% were anaemic. CD320 levels were significantly higher in anaemic mothers (414.09 ±10.75 pg/ml) when compared with cord blood (372.25 ± 7.81 pg/ml). CD320 levels were higher in both mother and cord blood with Vit B12 deficiency, however, were lower in mothers with active B12 deficiency. CD320 levels in cord blood were positively associated with haemoglobin (r value 0.8, p value 0.42) of mothers and negatively with serum folate (r value -0.15; p value 0.23), Vit B12(r value -0.21; p value0.28) and active Vit B12 levels (r value -0.25; p value0.03) in mothers. Conclusion: CD320 levels in cord blood positively correlated with haemoglobin of mothers and negatively with folic acid and B12 status. A negative trend (p=0.0024) was observed between maternal and neonatal CD320 levels.
Abuyaman, O., Andreasen, B.H., Kronborg, C., Vittinghus, E., Nexo, E. (2013). The soluble receptor for vitamin B12 uptake (sCD320) increases during pregnancy and occurs in higher concentration in urine than in serum. PLoS One,8(8):e73110. doi:10.1371/journal.pone.0073110
Adaikalakoteswari, A., Vatish M., Lawson A., Wood C., Sivakumar K., McTernan P.G., Webster C., Anderson N., Yajnik C.S., Tripathi G., et al. (2015). Low maternal vitamin B12 status is associated with lower cord blood HDL cholesterol in white Caucasians living in the UK. Nutrients,7:2401–2414. doi: 10.3390/nu7042401
Arendt, J.F., Nexo, E. (2012).Cobalamin related parameters and disease patterns in patients with increased serum cobalamin levels. PLOS ONE, 7: e45979 PubMed: 23029349
Arendt, J.F., Quadros, E.V., Nexo, E. (2012).Soluble transcobalamin receptor, sCD320, is present in human serum and relates to serum cobalamin – establishment and validation of an ELISA. Clin Chem Lab Med,50: 515–519.
Greibe,E., Andreasen, B.H., Lildballe, D.L., Morkbak, A.L., Hvas, A.M. et al.(2011). Uptake of cobalamin and markers of cobalamin status: a longitudinal study of healthy pregnant women. Clin Chem Lab Med, 49: 1877–1882.
Katre. P., Bhat, D., Lubree, H., Otiv, S., Joshi, S., Joglekar, C., Rush, E., Yajnik, C. (2010). Vitamin B12 and folic acid supplementation and plasma total homocysteine concentrations in pregnant Indian women with low B12 and high folate status. Asia Pac J Clin Nutr,19(3):335–43.
Koc, A., Kocyigit, A., Soran, M., Demir, N., Sevinc, E., Erel, O., Mil, Z. (2006). High frequency of maternal vitamin B12 deficiency as an important cause of infantile vitamin B12 deficiency in Sanliurfa province of Turkey. Eur. J. Nutr,45:291–297. doi: 10.1007/s00394-006-0598-7
Koebnick, C., Heins, U.A., Dagnelie, P.C., Wickramasinghe, S.N., Ratnayaka, I.D., Hothorn, T., Pfahlberg, A.B., Hoffmann, I., Lindemans, J., Leitzmann, C. (2002). Longitudinal concentrations of vitamin B(12) and vitamin B(12)-binding proteins during uncomplicated pregnancy. Clin Chem, 48(6 Pt 1):928-33.
Krafft, A., Huch, R., Breymann, C. (2003). Impact of parturition on iron status in non-anemic iron deficiency. Eur J Clin Invest,33:919–923.
Laxmaiah, A. (2015). Vitamin B12 and folic acid: significance in human health. Indian Pediatr, 52(5):380-381.
Lindgren, A., Kilander, A., Bagge, E., Nexo, E. (1999). Holotranscobalamin—a sensitive marker of cobalamin malabsorption. Eur J Clin Invest,29:321–9.
Milman, N., Byg, K.E., Bergholt, T., Eriksen, L., Hvas, A.M. (2006). Cobalamin status during normal pregnancy and postpartum: a longitudinal study comprising 406 Danish women.Eur J Haematol,76(6):521-5.
Moestrup, S.K., Birn, H., Fischer, P.B. et al. (1996). Megalin-mediated endocytosis of transcobalamin-vitamin-B12 complexes suggests a role of the receptor in vitamin-B12 homeostasis. Proc Natl Acad Sci U S A, 93(16):8612-8617. doi:10.1073/pnas.93.16.8612
Molloy, A.M., Kirke,P.N., Troendle, J.F., Burke, H., Sutton,M., Brody, L.C., et al.(2009). Maternal vitamin B12 status and risk of neural tube defects in a population with high neural tube defect prevalence and no folic acid fortification. Pediatrics,123:917–23. http://dx.doi.org/ 10.1542/peds.2008-1173.
Murphy, M.M., Molloy, A.M., Ueland, P.M., Fernandez-Ballart, J.D., Schneede, J., Arija, V., Scott, J.M. (2007). Longitudinal study of the effect of pregnancy on maternal and fetal cobalamin status in healthy women and their offspring. J. Nut, 7;137:1863–1867. doi: 10.1093/jn/137.8.1863
Muthayya, S., Dwarkanath, P., Mhaskar, M., Mhaskar, R., Thomas, A., Duggan, C., Fawzi, W.W., Bhat, S., Vaz, M., Kurpad, A. (2006). The relationship of neonatal serum vitamin B12 status with birth weight. Asia Pac. J. Clin. Nutr,15:538–543.
Nexo, E., Hvas, A.M., Bleie, O., Refsum, H., Fedosov, S.N., Vollset, S.E., Schneede, J., Nordrehaug, J.E., Ueland, P.M., et al. (2002). Holo-transcobalamin is an early marker of changes in cobalamin homeostasis. A randomized placebo-controlled study. Clin Chem,48:1768–71.
Nielsen, M.J., Rasmussen, M.R., Andersen, C.B., Nexo, E., Moestrup, S. K. (2012). Vitamin B12 transport from food to the body’s cells—a sophisticated, multistep pathway. Nat Rev Gastroenterol Hepatol,9: 345–354. doi:10.1038/nrgastro.2012.76
Pathak, P., Kapil, U., Yajnik, C.S., Kapoor, S.K., Dwivedi, S.N., Singh,R.(2007). Iron, folate, and vitamin B12 stores among pregnant women in a rural area of Haryana state, India. Food Nutr Bull,28(4):435-438.
Pepper, M.R., Black, M.M. (2011). B12 in fetal development. Semin Cell Dev Biol 2011; 22: 619–623. doi:10.1016/j.semcdb.2011.05.005.
Quadros, E.V., Nakayama, Y., Sequeira, J. M. (2009). The protein and the gene encoding the receptor for the cellular uptake of transcobalamin-bound Cobalamin. Blood ,113: 186–192. doi:10.1182/blood-2008-05-158949.
Rima, Obeid., Anne, L. Morkbak., Winfried, Munz., Ebba, Nexo., Wolfgang, Herrmann. (2006). The Cobalamin-Binding Proteins Transcobalamin and Haptocorrin in Maternal and Cord Blood Sera at Birth. Clinical Chemistry,52(2):263–269. https://doi.org/10.1373/clinchem.2005.057810
Rima, Obeid., Winfried, Munz., Monika, Jäger., Werner, Schmidt., Wolfgang, Herrmann. (2005). Biochemical indexes of the B vitamins in cord serum are predicted by maternal B vitamin status. AJCN ,82(1):133–139. https://doi.org/10.1093/ajcn/82.1.133
Van, den, Broek N.R., Letsky, E.A. (2000). Etiology of anemia in pregnancy in south Malawi. Am J Clin Nutr ,72(1):247S-256S.
Veena, S.R., Krishnaveni, G.V., Srinivasan, K., Wills, A.K., Muthayya, S., Kurpad, A.V., Yajnik, C.S., Fall, C.H. (2010). Higher maternal plasma folate but not vitamin B12 concentrations during pregnancy are associated with better cognitive function scores in 9- to 10- year-old children in South India. J Nutr,140(5):1014–22.
WHO. Haemoglobin concentrations for the diagnosis of anaemia and assessment of severity VMNIS | Vitamin and Mineral Nutrition Information System WHO/NMH/NHD/MNM/11.1 Microdeficiencies: Iron deficiency anaemia, http://www.who.int/ nutrition/topics/ida/en/
WHO. (1995). Physical Status: The Use and Interpretation of Anthropometry. World Health Organization; Geneva, Switzerland: p. 329. Report of a WHO Expert Committee.
Yajnik, C.S., Deshpande, S.S., Jackson, A.A., Refsum, H., Rao, S., Fisher, D.J., Bhat, D.S., Naik, S.S., Coyaji, K.J., Joglekar, C.V., Joshi, N., Lubree, H.G., Deshpande, V.U., Rege, S.S., Fall, C.H. (2008). Vitamin B12 and folate concentrations during pregnancy and insulin resistance in the offspring: the Pune Maternal Nutrition Study. Diabetologia,51(1):29–38.
Yetley, E.A., Pfeiffer, C.M., Phinney, K.W., Bailey, R.L., Blackmore, S., Bock, J.L., Brody, L.C., Carmel, R., Curtin, L.R., Durazo-Arvizu, R.A., et al. (2011). Biomarkers of vitamin B-12 status in NHANES: A roundtable summary. Am. J. Clin. Nutr,94:313S–321S. doi: 10.3945/ajcn.111.013243