Evaluation of Mast Cell and Eosinophil Count in Duodenal Mucosa- A Study on 69 Functional Dyspepsia Cases from Bangladesh

Journal of Health and Medical Sciences

ISSN 2622-7258

Published: 03 June 2019

Evaluation of Mast Cell and Eosinophil Count in Duodenal Mucosa- A Study on 69 Functional Dyspepsia Cases from Bangladesh

Madhusudan Saha, Shabanm Akhter, Mumit Sarkar, Shasanka Kumar Saha, Bimal Chandra Shil, ASM Nazmul Islam

North East Medical College, Rajshahi Medical College and Hospital, Dinajpur Medical College and Hospital, Sir Salimullah Medical College and Mitford Hospital, Saheed M Mansur Ali Medical College

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10.31014/aior.1994.02.02.37

Pages: 197-201

Keywords: Functional Dyspepsia, Duodenum, Eosinophil, Mast Cell

Abstract

The exact cause of functional dyspepsia is not known. This study was designed to investigate the association between duodenal infiltration of immune-mediating cells, namely eosinophils and mast cells with functional dyspepsia. Total of 69 patients presenting with symptoms of functional dyspepsia and matched healthy 42controls were included in this cross-sectional study. Tissue from duodenum was taken from both patients and controls, and the number of eosinophils and mast cells per five high power fields were counted. ‘t' test and chi square tests were done to find out the association of immune-mediating cells with functional dyspepsia. p value <0.05 was taken as significant. Duodenal eosinophil count ranges from 1-92 with mean 21.59 in cases and from 3-51 with mean 14.90 in controls. Eosinophil count was abnormally high among 29 (42.02%) and 08 (19.02%) among cases and controls, respectively, and the difference was significant (P=0.013).Mast cell density was seen among 56 cases and 23 controls from the same sample. Mast cell per 5 high power fields varied from 1-139 (mean 20.0536) and 3-57 (mean 20.86) were found in cases and controls groups, respectively (P=0.627).This study showed that functional dyspepsia is associated with a significant increase of eosinophils count in duodenum, but no significant association could be identified between of mast cell count in duodenum and functional dyspepsia.

References

  1. Collins, S.M. (1996).The immunomodulation of enteric neuromuscular function: Implications for motility and inflammatory disorders. Gastroenterology; 111, 1683–99.

  2. El-Serag, H.B., & Talley, N.J. (2004). Systematic review: the prevalence and clinical course of functional Dyspepsia.  Alimentary Pharmacology and Therapeutics, 19, 643–54.

  3. Friesen, C.A., Andre, L., Garola, R., Hodge, C.,& Roberts, C.( 2002). Activated duodenal mucosal eosinophils in children with dyspepsia: a pilot transmission electron microscopic study. J Pediatr Gastroenterol Nutr, 35, 329-33.

  4. Friesen, C.A., Sandridge, L., Andre, L., Roberts, C.C., & Abdel-Rahman, S.M. (2006). Mucosal eosinophilia and response to H1/H2 antagonist and cromolyn therapy in pediatric dyspepsia. ClinIcal Pediatrics, 45(2), 143–47.

  5. Friesen, C.A., Schurman, J.V., Colombo, J.M., & Abdel-Rahman, S.M.( 2013). Eosinophils and mast cells as therapeutic targets in pediatric functional dyspepsia. World Journal Gastrointestinal Pharmacology and Therapeutics, 4, 86-96.

  6. Futagami, S., Shindo, T., Kawagoe, T., Horie, A., Shimpuku, M. , Gudis, K., …….. Sakamoto, C. (2010). Migration of eosinophils and CCR2-/CD68-double positive cells into the duodenal mucosa of patients with postinfectious functional dyspepsia.  American Journal of Gastroenteroogyl, 105,1835–42.

  7. Futagami, S., Shimpuku, M., Yin, Y., Shindo, T., Kodaka, Y., Nagoya, H.,……… Sakamoto, C. (2011). Pathophysiology of functional dyspepsia. J Nippon Med Sch, 78, 281-5.

  8. Lee, K.J., & Tack, J. (2010). Duodenal Implications in the Pathophysiology of Functional Dyspepsia. J Neurogastroenterol Motil,  16: 251–257.

  9. Nyrop, K,A,, Palsson, O.S., Levy, R.L., Von Korff, M., Feld, A.D., Turner, M.J., & Whitehead, W.E. (2007). Costs of health care for irritable bowel syndrome, chronic constipation, functional diarrhoea and functional abdominal pain. Aliment pharmacol Ther,26, 237-48.

  10. Penston, J.G., & Pounder, R.E. (1996). A survey of dyspepsia in Great Britain. Aliment pharmacol Ther,10, 83-9.

  11. Powell, N., Walker, M.M., & Talley, N.J. (2010). Gastrointestinal eosinophils in health, disease and functional disorders. Nature Reviews Gastroenterology and Hepatology, 7(3), 146–56.

  12. Schurman, J.V., Singh, M., Singh, V., Neilan, N., & Friesen, C.A. (2010) Symptoms and subtypes in pediatric functional dyspepsia: relation to mucosal inflammation and psychological functioning. J Pediatr Gastroenterol Nutr, 51, 298-303.

  13. Rothenberg, M.E., Mishra, A., Collins, M.H., & Putnam, P.E. (2001). Pathogenesis and clinical features of eosinophilic esophagitis. J Allergy Clinical Immunol,108, 891-894.

  14.  

  15. Tack, J., Bisschops, R., & Sarnelli, G. (2004). Pathophysiology and treatment of functional dyspepsia. Gastroenterology,  127, 1239–55.

  16. Tack, J., Talley, N.J., Camilleri, M., Holtmann, G., Hu, P., Malagelada, J.R., & Stanghellini, V. (2006). Functional gastroduodenal disorders. Gastroenterology, 130(5),1466–79.

  17. Talley, N.J., Walker, M.M., Aro, P., Ronkainen, J., Storskrubb, T., Hindley, L.A., …. Agréuset, L. (2007). Non-ulcer dyspepsia and duodenal eosinophilia: an adult endoscopic population-based case-control study. Clinical Gastroenterology and Hepatology, 5(10), 1175-83.

  18. Theoharides, T.C., & Cochrane, D.E. (2004). Critical role of mast cells in inflammatory diseases and the effect of acute stress. J Neuroimmunol, 146, 1-12.

  19. Thumshirn, M. (2002).Pathophysiology of functional dyspepsia. Gut, 51(Suppl 1), i63–i66.

  20. Walker, M.M., & Talley, N.J. (2008). Functional Gastrointestinal Disorders and the Potential Role of Eosinophils. Gastroenterology Clinics of  North America, 37(2), 383–95.

  21. Walker, M.M., Talley, N.J., Prabhakar, M., Pennaneac’h, C.J., Aro, P., Ronkainen, J., ….. Agréuset, L. (2009). Duodenal mastocytosis, eosinophilia and intraepithelial lymphocytosis as possible disease markers in the irritable bowel syndrome and functional dyspepsia. Alimentary Pharmacology and Therapeutics, 29(7), 765-73.

  22. Walker, M.M., Salehian, S.S., Powell, N., Rajendran, A., Murray, C., Hoare, J., ….  Talley, N. (2010). Implications of eosinophilia in the normal duodenal biopsy—an association with allergy and functional dyspepsia. Alimentary Pharmacology and  Therapeutics, 31(11),  1229–36.

  23. Walker, M.M., Warwick, A., Ung, C., & Talley, N.J. (2011). The role of eosinophils and mast cells in intestinal functional disease. Curr Gastroenterol Rep, 13, 323-30.

  24. Zagari, R.M., Law, G.R., Fuccio, L.,  Cennamo, V., Gilthorpe, M.S., Forman, D., Bazzoli, F. (2010). Epidemiology of functional dyspepsia and subgroups in the Italian general population: an endoscopic study. Gastroenterology, 138,1302-11.

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