Journal of Health and Medical Sciences
Published: 08 April 2022
Immunohistochemical Expression of Cyclin D1 in Oral Lichen Planus
Mustafa M. Abdulhussain, Fawaz D. Alaswad
Mustansiriyah University (Iraq), Baghdad University (Iraq)
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Keywords: Immunohistochemistry, CyclinD1, Malignancy, Oral Lichen Planus
Background: Many studies have reported that a condition initially classified as oral lichen planus (OLP) has a variable chance of developing cancer progression over time, although these results are still questionable. Cyclin D1 controls the mitotic cell cycle's transition from G1 to S. Cyclin D1 has a significant function in carcinogenesis due to its critical involvement in cell cycle control. Cyclin D1 deregulation or overexpression may result in shortened G1 phase, enhanced cell replication, and decreased reliance on growth regulators, causing disruption in conventional cell cycle progression and the progression of cancer. The aim of the current study was to evaluate the expression of Cyclin D1 in histopathologically confirmed OLP samples and to predict the rate of malignant transformation in these lesions by comparing the level of expression with some clinical parameters. Materials and Methods: The immunohistochemical procedure was used to examine the expression of cyclin D1 on paraffin-embedded sections of (40) OLP lesions and (10) specimens of normal oral mucosa. We used the SPSS software version (18.0) to do the statistical analysis in this work. Results: There is a very significant difference between the people investigated (P = 0.01), which is statistically significant. CyclinD1 was found in all of the oral lichen planus cases in the research (100%), but it was also found (80%) in the normal oral mucosa. There was also no statistically significant difference between the clinical types of OLP and the Cyclin D1 scoring (P = 0.942), which revealed that the reticular type was the most frequent (7) in mild scoring, whereas the erosive type was the most frequent in severe scoring, which accounted for the majority of the cases. Conclusion: This research found that higher cyclinD1 expression increases the risk of cancer and many of the markers should be tested. Furthermore, larger sample numbers, improved designs, and gene-reduction studies should be performed. OLP patients should be continuously monitored for OSCC progression.
Abid AM, Merza MS. Immunohistochemical expression of Cyclin D1 and NF-KB p65 in oral lichen planus and oral squamous cellcarcinoma (Comparative study). J Bagh College Dentistry. 2014; 26(1):80-7.
Agha-Hosseini F, Mirzaii-Dizgah I, Abdollahi M, AkbariGillani N. Efficacy of IMOD in the treatment of oral lichen planus—efficacy of IMOD in oral lichen planus. Open J Stomatol 2011 Jun;1(2):13-17.
Aghbari SMH, Abushouk AI, Attia A, Elmaraezy A, Menshawy A, Ahmed MS, Elsaadany BA, Ahmed EM. Malignant transformation of oral lichen planus and oral lichenoid lesions: a meta-analysis of 20095 patient data. Oral Oncol 2017; 68:92- 102.
Bardellini E, Amadori F, Flocchini P, Bonadeo S, Majorana A. Clinicopathological features and malignant transformation of oral lichen planus: a 12-years retrospective study. Acta Odontol Scand 2013;71:834-40.
Bascones C, Gonzalez-Moles MA, Esparza G, Bravo M, Acevedo A, Gil-Montoya JA, et al. Apoptosis and cell cycle arrest in oral lichen planus Hypothesis on their possible influence on its malignant transformation. Arch Oral Biol. 2005; 50(10):873-81.
Bascones-Ilundain C, González-Moles MA, Campo-Trapero J, Gil-Montoya JA, Esparza-Gómez GC, Cano-Sánchez J, Bascones-Martínez A. No differences in caspase-3 and Bax expression in atrophic-erosive vs. reticular oral lichen planus. J Eur Acad Dermatol Venereol 2008;22:204-12.
Basnaker, S.S. and Satish, B.N.V.S., 2014. Cyclin d1 gene expression in oral mucosa of tobacco chewers”–an immunohistochemical study. Journal of Clinical and Diagnostic Research: JCDR, 8(5), p.ZC70.
Batool, S., Fahim, A., Qureshi, A., Anas, S., Qamar, N. and Kamran, S., 2019. Significance of Expression of Cyclin D as an Early Indicator in Dysplastic Transformation of Oral Mucosa in Tobacco Users. JPDA, 28(04).
Bombeccari GP, Guzzi G, Tettamanti M, Giannì AB, Baj A, Pallotti F, Spadari F. Oral lichen planus and malignant transformation: a longitudinal cohort study. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 2011 Sep;112(3):328-334
Carlos de Vicente J, Herrero-Zapatero A, Fresno MF, Lopez-Arranz JS. Expression of cyclin D1 and Ki-67 in squamous cell carcinoma of the oral cavity: clinicopathological and prognostic significance. Oral Oncol. 2002; 38(3):301- 8.
De Sousa FA, Paradella TC, Carvalho YR, Rosa LE. Comparative analysis of the expression of proliferating cell nuclear antigen, p53, bax, and bcl-2 in oral lichen planus and oral squamous cell carcinoma. Annals of Diagnostic Pathology. 2009 Oct 1; 13(5):308-12.
Dragomir LP, Simionescu C, Mărgăritescu CL, Stepan A, Dragomir IM, Popescu MR. P53, p16 and Ki67 immunoexpression in oral squamous carcinomas. Rom J Morphol Embryol. 2012 Jan 1; 53(1):89-93.
Fitzpatrick SG, Hirsch SA, Gordon SC. The malignant transformation of oral lichen planus and oral lichenoid lesions: a systematic review. J Am Dent Assoc. 2014; 145(1):45-56.
Gambichler T, Jaedicke V, Terras S. Optical coherence tomography in dermatology: technical and clinical aspects. Archives of dermatological research. 2011 Sep; 303(7):457-73.
Gandolfo S, Richiardi L, Carrozzo M, Broccoletti R, Carbone M, Pagano M, Vestita C, Rosso S, Merletti F. Risk of oral squamous cell carcinoma in 402 patients with oral lichen planus: a follow-up study in an Italian population. Oral Oncol 2004 Jan; 40(1):77-83.
Georgescu SR, Ene CD, Nicolae I, Mitran M, Musetescu A, Matei C, Rusu LC, Tampa M. Reflectometric analysis for identification of various pathological conditions associated with lichen planus. Rev Chim 2017; 68:1103-8.
Ghallab NA, Kasem RF, El-Ghani A, Fathy S, Shaker OG. Gene expression of miRNA-138 and cyclin D1 in oral lichen planus. Egyptian Dental Journal. 2017 Jan 1;63(1-January (Oral Medicine, X-Ray, Oral Biology & Oral Pathology)):625-38.
Giannetti L, Dello Diago AM, Spinas E. Oral lichen planus. J Biol Regul Homeost Agents 2018;32:391-5
Giuliani M, Troiano G, Cordaro M, Corsalini M, Gioco G, Lo Muzio L, Pignatelli P, Lajolo C. Rate of malignant transformation of oral lichen planus: a systematic review. Oral Dis 2019; 25:693-709.
González-Moles MA, Bascones-Ilundain C, Montoya JG, Ruiz-Avila I, and Delgado-Rodríguez M, Bascones-Martínez A. Cell cycle regulating mechanisms in oral lichen planus: molecular bases in epithelium predisposed to malignant transformation. Archives of oral biology. 2006 Dec 1; 51(12):1093-103.
Hadzi-Mihailovic M, Petrovic R, Raybaud H, Stanimirovic D, Koray MO. Expression and role of p53 in oral lichen planus patients. J BUON 2017; 22:1278-86.
Hwang EC, Choi SY, Kim JH. Malignant transformation of oral