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Asian Institute of Research, Journal Publication, Journal Academics, Education Journal, Asian Institute
Asian Institute of Research, Journal Publication, Journal Academics, Education Journal, Asian Institute

Journal of Health and Medical Sciences

ISSN 2622-7258

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open access

Published: 03 May 2023

Ketapang Leaf (Terminalia Catappa L.) Metabolite Profiling with Aquadest Fraction Ethanol Extract Using UPLC-MS

Asep Arifin Senjaya, Ni Made Sirat, I Gusti Ayu Raiyanti, Ni Ketut Ratmini

Poltekkes Kemenkes Denpasar, Indonesia

journal of social and political sciences
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Pages: 8-15

Keywords: Ketapang, Metabolite, Aquadest, UPLC-MS


Ketapang leaves (Terminalia catappa L.) are known for its traditional medicinal function. Ketapang leaves contain saponins, alkaloids, tannins, flavonoids, and triterpenoids. In general, polar solvent can increase the production of Ketapang leaves’ active compound. Utilization of a plant as herbal medicine is based on the presence of chemical compounds that have certain pharmacological effects. Therefore, metabolite profiling is needed. This is intended to understand the chemical compounds of Ketapang leaves. The objective of this study is to analyze the metabolite profile of chemical compounds and major compounds contained in the aquadest fraction ethanol extract using the UPLC-MS instrument. The results of the interpretation of the analysis of the compound content using UPLC-MS showed that there are 19 compounds in the ethanol extract of the aquadest fraction of Ketapang leaves. The major compound in the ethanol extract of the aqueous fraction of Ketapang leaves is Pelargonidin 3-O-glucoside with an iFit percentage of 97.56%.


  1. Allyn, O. Q., Kusumawati, E., & Nugroho, R. A. (2018).  Antimicrobial activity of terminalia catappa brown leaf extracts against  staphylococcus aureus ATCC 25923 and Pseudomonas aeruginosa ATCC 27853  [version 1; peer review: 2 approved, 1 not approved]. F1000Research, 7.

  2. Attwa, M. W., AlRabiah, H., Mostafa, G. A. E., & Kadi,  A. A. (2023). Development of an LC-MS/MS Method for Quantification of  Sapitinib in Human Liver Microsomes: In Silico and In Vitro Metabolic  Stability Evaluation. Molecules, 28(5).

  3. Chawla, G., & Ranjan, C. (2016). Principle,  Instrumentation, and Applications of UPLC: A Novel Technique of Liquid  Chromatography. Open Chemistry Journal, 3(1), 1–16.

  4. Chuah, A. L., & Pisar, M. M. (2010). Antioxidant and  anti-inflammatory activities of extracts of betel leaves (Piper betle) from  solvents with different polarities. In Article in Journal of Tropical  Forest Science.

  5. Dembek, M., & Bocian, S. (2022). Stationary Phases for  Green Liquid Chromatography. In Materials (Vol. 15, Issue 2). MDPI.

  6. Dembitsky, V. M. (2021). In Silico Prediction of Steroids  and Triterpenoids as Potential Regulators of Lipid Metabolism. In Marine  Drugs (Vol. 19, Issue 11). MDPI.

  7. Ergün, F. (2022). The Effects of Drying Methods on Total Phenolic  and Flavonoid Substances and Antioxidant Capacity of Redstem Filaree (Erodium  Cicutarium). Applied Ecology and Environmental Research, 20(1),  499–509.

  8. Janporn, S., Ho, C. T., Chavasit, V., Pan, M. H., Chittrakorn,  S., Ruttarattanamongkol, K., & Weerawatanakorn, M. (2015). Physicochemical  properties of Terminalia catappa seed oil as a novel dietary lipid source. Journal  of Food and Drug Analysis, 23(2), 201–209.

  9. Katiki, L. M., Gomes, A. C. P., Barbieri, A. M. E.,  Pacheco, P. A., Rodrigues, L., Veríssimo, C. J., Gutmanis, G., Piza, A. M.,  Louvandini, H., & Ferreira, J. F. S. (2017). Terminalia catappa: Chemical  composition, in vitro and in vivo effects on Haemonchus contortus. Veterinary  Parasitology, 246, 118–123.

  10. Ladele, B., Kpoviessi, S., Ahissou, H., Gbenou, J.,  Kpadonou-Kpoviessi, B., Mignolet, E., Hérent, M. F., Bero, J., Larondelle, Y.,  Quetin-Leclercq, J., & Moudachirou, M. (2016). Chemical composition and  nutritional properties of Terminalia catappa L. oil and kernels from Benin. Comptes  Rendus Chimie, 19(7), 876–883.

  11. Marcińczyk, N., Gromotowicz-Popławska, A., Tomczyk, M.,  & Chabielska, E. (2022). Tannins as Hemostasis Modulators. In Frontiers  in Pharmacology (Vol. 12). Frontiers Media S.A.

  12. Milovanova-Palmer, J., & Pendry, B. (2018). Is there a  role for herbal medicine in the treatment and management of periodontal  disease? Journal of Herbal Medicine, 12, 33–48.

  13. Muthulakshmi, G., & Neelanarayanan, P. (2021).  Evaluation of Antimicrobial Activities of Terminalia catappa Leaves ’ Extracts  against Bacterial and Fungal Pathogens. Indian Journal of Natural Sciences,  11(64), 976–997.

  14. Nugroho, R. A., Manurung, H., Saraswati, D., Ladyescha, D.,  & Nur, F. M. (2016). The Effects of Terminalia catappa L. Leaves Extract  on the Water Quality Properties, Survival and Blood Profile of Ornamental fish  (Betta sp) Cultured. Biosaintifika: Journal of Biology & Biology  Education, 8(2), 241.

  15. Ola, P. D., Sandri, M. I., Ola, A. R. B., & Kadang, L.  (2020). Determination of Total Tanin Contents of Terminalia Catappa, L. Leaf  Extract and Test of Its Ability as A Complextion Agent of Fe (III). Chem.  Notes, 1(2), 94–107.

  16. Olukotun, A. B., Bello, I. A., & Oyewale, O. A. (2018).  Phytochemical and anthelmintic activity of Terminalia catappa (Linn)  leaves. Journal of Applied Sciences and Environmental Management, 22(8),  1343.

  17. Praptiwi, P., Dewi Wulansari, Ahmad Fahoni, Noto Hartono,  Rossi Novita, Alfridsyah, & Andria Agusta. (2020). Phytochemical  screening, antibacterial and antioxidant assessment of Leuconotis eugenifolia  leaf extract. Nusantara Bioscience, 12(1).

  18. Punwong, P., Juprasong, Y., & Traiperm, P. (2017).  Effects of an oil spill on the leaf anatomical characteristics of a beach  plant (Terminalia catappa L.). Environmental Science and Pollution Research,  24(27), 21821–21828.

  19. Sani, L., Ajibola, V. O., & Abechi, S. E. (2019).  Effect of degumming and catalyst type on physiochemical and biodiesel  properties of tropical-almond (Terminalia catappa) seed oil. Journal  of Applied Sciences and Environmental Management, 22(12), 1909.

  20. Serwecińska, L. (2020). Antimicrobials and  antibiotic-resistant bacteria: A risk to the environment and to public health.  Water  (Switzerland), 12(12).

  21. Tampemawa, P. V, Pelealu, J. J.,  Kandou, F. E., & Biologi Fakultas MIPA Universitas Sam Ratulangi, J.  (2016). Uji Efektivitas Ekstrak Daun  Ketapang (Terminalia catappa L.) Terhadap Bakteri Bacillus amyloliquefaciens  (Effectiveness Test of Ketapang Leaf Extract (Terminalia catappa L.) Against  Bacillus amyloliquefaciens Bacteria). In PHARMACONJurnal Ilmiah  Farmasi-UNSRAT (Vol. 5, Issue 1).

  22. Telrandhe, U. B., Lokhande, R. R.,  Lodhe, V. N., Kosalge, S. B., Parihar, S., & Sharma, D. (2021). Review on Herbal Drugs used in  Dental Care Management. Asian Journal of Pharmaceutical Research and  Development, 9(6), 71–79.

  23. Ullah, A., Munir, S., Badshah, S. L., Khan, N., Ghani, L.,  Poulson, B. G., Emwas, A. H., & Jaremko, M. (2020). Important flavonoids  and their role as a therapeutic agent. In Molecules (Vol. 25, Issue  22). MDPI AG.

  24. Valizadeh, M., Alimohammadi, F., Azarm, A., Pourtaghi, Z.,  Derakhshan barjoei, M. moein, Sabri, H., Jafari, A., Arabpour, Z., Razavi, P.,  Mokhtari, M., & Deravi, N. (2021). Uses of soybean isoflavonoids in  dentistry: A literature review. In Journal of Dental Sciences.  Association for Dental Sciences of the Republic of China.

  25. Zhao, Y. Y., & Lin, R. C. (2014). UPLC-MSE application  in disease biomarker discovery: The discoveries in proteomics to metabolomics.  In Chemico-Biological Interactions (Vol. 215, Issue 1, pp. 7–16).  Elsevier Ireland Ltd.