The Comparison of Local to Commercial Bacillus thuringiensis Against Aedes aegypti Larvae

Journal of Health and Medical Sciences

ISSN 2622-7258

Published: 13 November 2018

The Comparison of Local to Commercial Bacillus thuringiensis Against Aedes aegypti Larvae

Lia Faridah, Sofia Salsabila, Nisa Fauziah

Universitas Padjadjaran, Indonesia

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10.31014/aior.1994.01.01.4

Abstract

DHF as vector-borne disease become a problem in the world. The most effective way for suppressing the incidence is killing the vector, Aedes aegypti. One of the ways is using a natural insecticide, local Bacillus thuringiensis (Bt). The study aimed to determine the efficacy of local Bt against Aedes aegypti larvae. The study was conducted by using an experimental design with 1.250 3rd and 4th instar larvae Aedes aegypti as a sample. The study consist of 19 groups, 6 groups of local Bt from Curugdago, 6 groups of local Bt from Setiabudi, 6 groups of Bactivec® (commersial Bt), and a group without larvicide. Each group consist of 25 larvae included in the glass of media with concentrations of 0.04; 0.03; 0.02; 0.01; 0.008; and 0.005 ppm. An observation was made after 24 hours of exposure by counting the number of dead larvae. Data were analyzed using probit analysis to determine LC50, LC90, and LC99. The study was conducted in Unpad Faculty of Medicine Microbiology and Parasitology Laboratory. The result of the study showed that local Bt could kill Aedes aegypti larvae with LC50, LC90, LC99 is 0.043; 0.153; 0.436 for local Bt from Curugdago and 0.104; 0.21; 0.809 for local Bt from Setiabudi. That value is higher if compared with Bactivec®, therefore, it can be concluded that local Bt has larvicide effect against Aedes aepgyti larvae, however, there is not as effective as commercial Bt.

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