Journal of Health and Medical Sciences
ISSN 2622-7258
Published: 19 September 2022
Antibacterial Effects of Various Concentrations of Natural Ingredients Snail Mucus (Achatina fulica) Against Inhibition Zones of Fusobacterium nucleatum Causes Periodontitis In Vitro
Swastini IGAAP, Ni Nengah Sumerti
Politeknik Kesehatan Kemenkes Denpasar, Indonesia
Download Full-Text Pdf
10.31014/aior.1994.05.03.235
Pages: 262-267
Keywords: Antibacterial, Snail Slime, Fusobacterium Nucleatum
Abstract
Periodontitis is a microorganism that invades the subgingival area, thereby triggering an inflammatory response of the periodontal tissue. Fusobacterium nucleatum is one of the most abundant gram-negative bacilli colonizing the subgingival plaque and closely associated with periodontal disease. The aim of study was to calculate the inhibition of snail mucus against Fusobacterium nucleatum bacteria with concentrations of 12.5%, 25%, 50%, and 100%. This study used a laboratory experimental test method and was conducted at the Oral Biology Laboratory, Faculty of Dentistry, Airlangga University. The steps were performed are: plant germ stock with sterile osse on BHI broth media then incubate for 48 hours, observe the turbidity of the germs then standardize with mc farland standard 0.5, plant germs on Hilton Muller agar media with the spreading technique, treat the test sample on sterile paper disk as much as 0, 01 ml with a sterile micropipette, then paste it on the surface of the media so that it is then incubated for 48 hours. Then, observe and measure the diameter of the clear zone. The results show that the average inhibition in the control group is 25.65 mm, and the snail mucus group is 12.5%, 0 mm, 25%, 12.40 mm, 50%, 16.70 mm and 100% 19.60 mm. There is a significant difference in the diameter of the inhibitory power between the treatment groups. The conclusion of the antibacterial effect of natural ingredients of snail mucus from the 25% treatment showed strong criteria, and the greatest inhibitory power was the concentration of 100%.
References
Berniyanti, T., & Suwarno. (2010). Characteristics of Local Isolated Snail Slime Protein (Achasin) as Anti-Bacterial. Factor: Veterinary Media, 23(3), 139–144.
Carranza, F. A., Takei, H. H., & Newman, M. G. (2012). Clinical Periodontology (11th ed.). Saunders-Elsevier.
Davis, W. W., & Stout, T. R. (1971). Disc plate method of microbiological antibiotic assay. II. Novel procedure offering improved accuracy. Applied Microbiology, 22(4), 666–670. https://doi.org/10.1128/aem.22.4.666-670.1971
Evan Wijaksana, I. K. (2016). Infectobesity Dan Periodontitis: Hubungan Dua Arah Obesitas Dan Penyakit Periodontal [Bidirectional Relationship of Obesity and Periodontal Disease]. ODONTO : Dental Journal, 3(1), 67. https://doi.org/10.30659/odj.3.1.67-73
Han, Y. W., Shi, W., Huang, G. T. J., Kinder Haake, S., Park, N. H., Kuramitsu, H., & Genco, R. J. (2000). Interactions between periodontal bacteria and human oral epithelial cells: Fusobacterium nucleatum adheres to and invades epithelial cells. Infection and Immunity, 68(6), 3140–3146. https://doi.org/10.1128/IAI.68.6.3140-3146.2000
How, K. Y., Song, K. P., & Chan, K. G. (2016). Porphyromonas gingivalis: An overview of periodontopathic pathogen below the gum line. Frontiers in Microbiology, 7(FEB), 1–14. https://doi.org/10.3389/fmicb.2016.00053
Liu, P., Liu, Y., Wang, J., Guo, Y., Zhang, Y., & Xiao, S. (2014). Detection of fusobacterium nucleatum and fada adhesin gene in patients with orthodontic gingivitis and non-orthodontic periodontal inflammation. PLoS ONE, 9(1), 3–7. https://doi.org/10.1371/journal.pone.0085280
Manson, J. D. (2013). Buku ajar periodonti. Hipokrates.
Merglova, V., Koberova-Ivancakova, R., Broukal, Z., & Dort, J. (2014). The presence of cariogenic and periodontal pathogens in the oral cavity of one-year-old infants delivered pre-term with very low birthweights: A case control study. BMC Oral Health, 14(1), 1–8. https://doi.org/10.1186/1472-6831-14-109
Newman, M. G., Carranza, F. A., Bulkasez, J., Quirynen, M., Teughels, W., & Haake, S. K. (2012). Microbiology of Periodontal Disease in Carranza’s Clinical Periodontology (10th ed.). Saunders Elseviers.
Park, J., Shokeen, B., Haake, S. K., & Lux, R. (2016). Characterization of Fusobacterium nucleatum ATCC 23726 adhesins involved in strain-specific attachment to Porphyromonas gingivalis. International Journal of Oral Science, 8(3), 138–144. https://doi.org/10.1038/ijos.2016.27
Perry, D. A., & Beemsterboer, P. L. (2007). Periodontology for the dental hygienist (3rd ed.). Saunders-Elsevier.
Samaranayake, L. (2012). Essential microbiology for Dentistry (4th ed.). Elsevier.
Scannapieco, F. A., & Dongari-Bagtzoglou, A. (2021). Dysbiosis revisited: Understanding the role of the oral microbiome in the pathogenesis of gingivitis and periodontitis: A critical assessment. Journal of Periodontology, 92(8), 1071–1078. https://doi.org/10.1002/JPER.21-0120
Swastini, I. (2011). Topical Administration of Snail Mucus Cure Gingivitis Grade 3 Faster Due to Calculus than 10% Povidone Iodine. Udayana University.
Swastini, I. (2019). Provision of snail mucus can result in lower levels of malondialdehyde, vascular endothelial growth factor, transforming growth factor -1 and a higher number of fibroblasts in wistar rats with periodontitis. Udayana University.