Journal of Health and Medical Sciences
Published: 22 August 2023
Identification of Candida Isolates from Cancer Patients Using Multiplex PCR
S. C. Ganguli, W. A. S. Wijendra, P. N. Dasanayaka, R. Ramesh, A. G. G. Kaushalya, S. Gunasekara
Medical Research Institute, University of Sri Jayewardenepura, Apkesha Hospital
Download Full-Text Pdf
Keywords: Multiplex PCR, Cancer Patients, Candida
Aim of this study was to identify the Candida isolates from Cancer Hospital Maharagama, Sri Lanka by using Multiplex PCR (polymerase chain reaction) method. In this study, Multiplex PCR was used to identify Candida isolates from cancer patients at Apeksha Hospital, Sri Lanka. In multiplex PCR, 18 specific primers (nine primer pairs) were separated into three groups for multiplex PCR (PS I, PS II and PS III). Different sized PCR products corresponding to each Candida species were produced by PCR using the primer mixes. Among 52 Candida isolates, 49 were identified using the multiplex PCR procedure. Candida tropicalis was found to be the most prevalent species (38%), followed by Candida parapsilosis (31%), Candida albicans (13%), Candida glabrata (8%) and Candida krusei (4%). The study concludes that multiplex PCR is a better approach for the identification of Candida species and recommends the use of it for clinical and diagnostic purposes and for research purposes.
Arastehfar, A., Fang, W., Pan, W., Liao, W., Yan, L. and Boekhout, T. (2018). Identification of nine cryptic species of Candida albicans, C. glabrata, and C. parapsilosis complexes using one-step multiplex PCR. BMC Infectious Diseases 18, pp. 1–9.
Huang WM. (1996). Bacterial diversity based on type II DNA topoisomerase genes. Ann Rev Genet 30: 79– 107.
Kanbe, T., Horii, T., Arishima, T., Ozeki, M. and Kikuchi, A. (2002). PCR-based identification of pathogenic Candida species using primer mixes specific to CandidaDNA topoisomerase II genes. Yeast, 19(11), pp. 973–989.
Kato, M., Ozeki, M., Kikuchi, A. and Kanbe, T. (2001). Phylogenetic relationship and mode of evolution of yeast DNA topoisomerase II gene in the pathogenic Candida species’, Gene, 272(1-2), pp. 275–281
Kumar A. (2018). A fungus among us: The emerging opportunistic pathogen Candida tropicalis and PKA signaling. Virulence. 9(1), pp. 659–661.
Lin, C.-Y. et al. (2007). Assessment of Candida glabrata Strain Relatedness by Pulsed-Field Gel Electrophoresis and Multilocus Sequence Typing. Journal of Clinical Microbiology, 45(8), pp. 2452–2459.
Mahmoudi Rad, M. et al.(2012) ‘Identification of Candida Species Associated with Vulvovaginal Candidiasis by Multiplex PCR’, Infectious Diseases in Obstetrics and Gynecology, 2012, pp. 1–5.
Sigera S, Kudavidanage S, Jayasekera P, Jayawardena M, Perera P, Thabrew H and Malkanthi M. (2019). Candida blood stream infections in Sri Lanka: a retrospective evaluation by the National Mycology Reference Laboratory.
Trofa D, Gacser A and Nosanchuk J D. (2008). Candida parapsilosis, an emerging fungal pathogen. Clinical Microbiology Reviews. 21(4), pp.606–625.