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Asian Institute of Research, Journal Publication, Journal Academics, Education Journal, Asian Institute
Asian Institute of Research, Journal Publication, Journal Academics, Education Journal, Asian Institute

Journal of Health and Medical Sciences

ISSN 2622-7258

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open access

Published: 09 December 2023

Ethanol Extract of Pomelo Peel to Prevent Dental Caries in Extracted Teeth In-Vitro

I Made Budi Artawa, I Gede Surya Kencana, I Nyoman Gejir

Poltekkes Kemenkes Denpasar, Indonesia

journal of social and political sciences
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Pages: 177-184

Keywords: Caries Teeth, Citrus Maxima, In Vitro Extracted Teeth, Pomelo Peel Extract, S. Mutans


Dental caries is caused by the bacteria Streptococcus mutans. It is said that S. mutans can be reduced by the action of toothbrushing with mouthwashes. However, chemical mouthwashes bring adverse effect on the teeth such as tooth staining, microbiota ecology changes, and mucosa lesions. Natural mouthwashes have become the alternative solution. For instance, the ability of ethanol extract from Pomelo peel to inhibit the growth of S. mutans bacteria can be used to prevent dental caries. This study aims to determine the effectiveness of ethanol extract of Pomelo peel in preventing dental caries in extracted teeth in vitro. The research design was quasi-experimental with a number of samples is 30. Six treatments were carried out, each treatment consisted of five samples consisting of bacterial culture, ethanol extract of Pomelo peel 20%, 50%, 75%, negative control, and positive control plus teeth and substrate. Observations were recorded and analyzed using Univariate Multi-Way Analysis of Variance (ANOVA). The results of the study showed that the occurrence of dental caries after receiving ethanol extract treatment from Pomelo peel is at least in the 75% concentration group, namely 0 teeth (0%), at the 25% concentration, all teeth experienced caries (100%), and in the 50% concentration group, dental caries occurred 67%. Statistically, grapefruit peel extract with a concentration of 75% significantly inhibits the process of tooth decay in conditions of white spots, black spots and holes. In conclusion, 75% concentration of Pomelo peel ethanol extract is effective in preventing dental caries in extracted teeth in-vitro.


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