Ni Made Sirat, Asep Arifin Senjaya
Poltekkes Kemenkes Denpasar, Indonesia
Complications in tooth extraction are such as bleeding, fracture, dry socket, swelling, shock, and several other complications. Ketapang (Terminalia catappa L.) is known to contain flavonoids which play a role in wound healing. The flavonoid content of T. Catappa L. can accelerate wound healing in the angiogenesis process by stimulating vascular endothelial growth factors. This study aims to examine the chemical sub-element of T. Catappa L. leaves extract and its toxicity by Lethal Dose (LD50) test in mice. This research was experiment in a laboratory of making ethanol extract from T. Catappa L. leaves. The LD50 test of ethyl acetate fractionated T. Catappa L. leaves extract was carried out on 20 mice divided into 5 treatment groups, namely 1, 2, 4, 8, and 16 g/kg body weight (BW). Graphical analysis of the results of the Liquid chromatography-mass spectrometry (LCMS) test was carried out to determine the detected chemical compounds and histopathology images were obtained. T. Catappa L. has tannin and flavonoid compounds. The results of the LD50 test show that no mice were poisoned and died. Histopathological tests on mice that were given a dose of 16gr/kg BW show that the liver has diffuse hydrophilic degeneration, while the normal kidneys have no abnormalities. Ethyl acetate fractionated ethanol extract of T. Catappa L. leaves is categorized as non-toxic. Based on the content of tannin and flavonoid compounds, it is suggested to use T. Catappa L. leaves as an anti-bleeding agent after tooth extraction.
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